faculty

Toshio Tsukiyama

ttsukiya@fredhutch.org

Fred Hutch, 

Gene Expression, Cell Cycle & Chromosome Biology (Area Director)

Genetics, Genomics & Evolution

chromatin regulation for cell division and cell-cycle control

Faculty Contact Information

Building: A1 Room: A1-175 Box: A1-162 Phone: 206-667-4996 http://research.fhcrc.org/tsukiyama/en.html

Lab Information

Location: Fred Hutchinson Cancer Research Center Building: A1 Room: A1-175 Box: A1-162 Phone: 206-667-4996 http://research.fhcrc.org/tsukiyama/en.html

Accepting Students For:

Rotation, Autumn
Rotation, Spring
Rotation, Summer
Rotation, Winter
Permanent

Publications

Condensin-Dependent Chromatin Compaction Represses Transcription Globally during Quiescence.

Swygert SG, Kim S, Wu X, Fu T, Hsieh TH, Rando OJ, Eisenman RN, Shendure J, McKnight JN, Tsukiyama T.

Molecular cell. 2019; 73(3):533-546.e4. NIHMSID: NIHMS1513147

PubMed [journal]
PMID:
30595435
PMCID:
PMC6368455

Isw2 and Ino80 chromatin remodeling factors regulate chromatin, replication, and copy number at the yeast ribosomal DNA locus

Cutler SL.

BioXiv. 2018 March 30;

My Bibliography [journal]

Isw2 and Ino80 chromatin remodeling factors regulate chromatin, replication, and copy number at the yeast ribosomal DNA locus.

Cutler SL, Lee LJ, Tsukiyama T.

bioRxiv [Preprint]. 2018 March 30. Available from: https://www.biorxiv.org/content/early/2018/03/30/291971. DOI: https://doi.org/10.1101/291971.

My Bibliography [Interim Product]

Nucleosome occupancy as a novel chromatin parameter for replication origin functions.

Rodriguez J, Lee L, Lynch B, Tsukiyama T.

Genome research. 2017; 27(2):269-277.

PubMed [journal]
PMID:
27895110
PMCID:
PMC5287232

Sequence-targeted nucleosome sliding in vivo by a hybrid Chd1 chromatin remodeler.

McKnight JN, Tsukiyama T, Bowman GD.

Genome research. 2016; 26(5):693-704.

PubMed [journal]
PMID:
26993344
PMCID:
PMC4864466

Research Summary

In eukaryotic cells, DNA is packaged into chromatin, which allows compact storage of the genome, but limits the access of DNA binding proteins to their targets. As a result, chromatin structure affects virtually all DNA-dependent processes and chromatin regulators are frequently mutated in cancer. However, how chromatin regulation affects cell division and cell-cycle is not well unserstood. We are especially interested in cell quiescence, and are investigating how chromatin regulation affects the entry, maintenance and exit from quiescence.