faculty

Kelly Lee

kklee@uw.edu

University of Washington, 

Biophysical and Structural Biology (Area Director)

Microbiology, Infection & Immunity

VIRUS STRUCTURE, DYNAMICS AND FUNCTION. MEMBRANE FUSION.

Faculty Contact Information

Building: Health Sciences Building Room: H-172J Box: 357610 Phone: 206-616-3972 http://faculty.washington.edu/kklee/Welcome.html

Lab Information

Location: University of Washington Building: Health Sciences Building Room: H-053/H-059 Box: 357610 Phone: 206-616-3496 http://faculty.washington.edu/kklee/Welcome.html

Accepting Students For:

Rotation, Autumn
Rotation, Spring
Rotation, Summer
Rotation, Winter
Permanent

Publications

Tracking Higher Order Protein Structure by Hydrogen-Deuterium Exchange Mass Spectrometry.

Benhaim M, Lee KK, Guttman M.

Protein and peptide letters. 2019; 26(1):16-26. NIHMSID: NIHMS948124

PubMed [journal]
PMID:
30543159
PMCID:
PMC6386625

Single-Molecule Analysis of a Viral Fusion Protein Illuminates a Fusion-Active Intermediate State.

Benhaim M, Lee KK.

Cell. 2018; 174(4):775-777.

PubMed [journal]
PMID:
30096307

Nucleoside-modified mRNA vaccines induce potent T follicular helper and germinal center B cell responses.

Pardi N, Hogan MJ, Naradikian MS, Parkhouse K, Cain DW, Jones L, Moody MA, Verkerke HP, Myles A, Willis E, LaBranche CC, Montefiori DC, Lobby JL, Saunders KO, Liao HX, Korber BT, Sutherland LL, Scearce RM, Hraber PT, Tombácz I, Muramatsu H, Ni H, Balikov DA, Li C, Mui BL, Tam YK, Krammer F, Karikó K, Polacino P, Eisenlohr LC, Madden TD, Hope MJ, Lewis MG, Lee KK, Hu SL, Hensley SE, Cancro MP, Haynes BF, Weissman D.

The Journal of experimental medicine. 2018; 215(6):1571-1588.

PubMed [journal]
PMID:
29739835
PMCID:
PMC5987916

Dissection of Epitope-Specific Mechanisms of Neutralization of Influenza Virus by Intact IgG and Fab Fragments.

Williams JA, Gui L, Hom N, Mileant A, Lee KK.

Journal of virology. 2018; 92(6).

PubMed [journal]
PMID:
29263254
PMCID:
PMC5827376

Methods in Molecular Biology

Gui L, Lee KK.

Yamauchi Y, editor. New York: Springer; 2018. Influenza Virus-Liposome Fusion Studies using Fluoresence Dequenching and Cryo-Electron Tomography

My Bibliography [book]

Research Summary

Viruses undergo dynamic structural reorganizations at many critical stages of their life cycles including during host cell invasion, membrane fusion, genome expulsion, assembly, and cell egress. The changes often involve concerted changes among hundreds of protein components and in the case of enveloped viruses, membranes as well. We use biophysical, structural, and biochemical techniques including cryo-EM, hydrogen/deuterium-exchange with mass spectrometry (HDX-MS), and small-angle X-ray scattering (SAXS) to understand these dynamic processes.