Karol Bomsztyk
karolb@u.washington.eduUniversity of Washington,
Cancer Biology
Cell Signaling & Cell/Environment Interactions
Gene Expression, Cell Cycle & Chromosome Biology
Epigenetic alteration in disease
Faculty Contact Information
Lab Information
Accepting Students For:
Publications
The following publications were retrieved from PubMed:
Bomsztyk K, Mar D, Wang Y, Denisenko O, Ware C, Frazar CD, Blattler A, Maxwell AD, MacConaghy BE, Matula TJ.
Nucleic Acids Res. 2019 Mar 30;
Sharifian R, Okamura DM, Denisenko O, Zager RA, Johnson A, Gharib SA, Bomsztyk K.
Sci Rep. 2018 Dec 14; 1(8)17870
Haynes P, Bomsztyk K, Miller DG.
Epigenetics Chromatin. 2018 Aug 20; 1(11)47
Chromatin changes trigger laminin genes dysregulation in aging kidneys.
Denisenko O, Mar D, Trawczynski M, Bomsztyk K.
Aging (Albany NY). 2018 May 29; 5(10)1133-1145
DNA methylation yields epigenetic clues into the diabetic nephropathy of Pima Indians.
Bomsztyk K, Denisenko O, Wang Y.
Kidney Int. 2018 Jun; 6(93)1272-1275
Engineering a multicellular vascular niche to model hematopoietic cell trafficking.
Kotha SS, Hayes BJ, Phong KT, Redd MA, Bomsztyk K, Ramakrishnan A, Torok-Storb B, Zheng Y.
Stem Cell Res Ther. 2018 Mar 23; 1(9)77
Mikula M, Skrzypczak M, Goryca K, Paczkowska K, Ledwon JK, Statkiewicz M, Kulecka M, Grzelak M, Dabrowska M, Kuklinska U, Karczmarski J, Rumienczyk I, Jastrzebski K, Miaczynska M, Ginalski K, Bomsztyk K, Ostrowski J.
Nucleic Acids Res. 2016 Dec 1; 21(44)10150-10164
Denisenko O, Lucas ES, Sun C, Watkins AJ, Mar D, Bomsztyk K, Fleming TP.
Biochim Biophys Acta. 2016 Jul; 7(1859)906-13
SYSTEM-WIDE MAPPING OF ACTIVATED CIRCUITRY IN EXPERIMENTAL SYSTEMIC INFLAMMATORY RESPONSE SYNDROME.
Gharib SA, Mar D, Bomsztyk K, Denisenko O, Dhanireddy S, Liles WC, Altemeier WA.
Shock. 2016 Feb; 2(45)148-56
Zamiri B, Mirceta M, Bomsztyk K, Macgregor RB Jr, Pearson CE.
Nucleic Acids Res. 2015 Nov 16; 20(43)10055-64
Mikula M, Rubel T, Karczmarski J, Statkiewicz M, Bomsztyk K, Ostrowski J.
Proteome Sci. 2015; (13)23
Morris DP, Lei B, Longo LD, Bomsztyk K, Schwinn DA, Michelotti GA.
PLoS One. 2015; 8(10)e0134442
Mar D, Gharib SA, Zager RA, Johnson A, Denisenko O, Bomsztyk K.
Kidney Int. 2015 Oct; 4(88)734-44
Bomsztyk K, Mar D, An D, Sharifian R, Mikula M, Gharib SA, Altemeier WA, Liles WC, Denisenko O.
Crit Care. 2015 May 11; (19)225
M2 Macrophage Polarization Mediates Anti-inflammatory Effects of Endothelial Nitric Oxide Signaling.
Lee WJ, Tateya S, Cheng AM, Rizzo-DeLeon N, Wang NF, Handa P, Wilson CL, Clowes AW, Sweet IR, Bomsztyk K, Schwartz MW, Kim F.
Diabetes. 2015 Aug; 8(64)2836-46
Losartan reverses permissive epigenetic changes in renal glomeruli of diabetic db/db mice.
Reddy MA, Sumanth P, Lanting L, Yuan H, Wang M, Mar D, Alpers CE, Bomsztyk K, Natarajan R.
Kidney Int. 2014 Feb; 2(85)362-73
Epigenetic alterations in acute kidney injury.
Bomsztyk K, Denisenko O.
Semin Nephrol. 2013 Jul; 4(33)327-40
Bomsztyk K, Flanagin S, Mar D, Mikula M, Johnson A, Zager R, Denisenko O.
PLoS One. 2013; 7(8)e70322
Activation of Wnt/β-catenin signaling increases apoptosis in melanoma cells treated with trail.
Zimmerman ZF, Kulikauskas RM, Bomsztyk K, Moon RT, Chien AJ.
PLoS One. 2013; 7(8)e69593
Mikula M, Bomsztyk K, Goryca K, Chojnowski K, Ostrowski J.
J Biol Chem. 2013 Aug 23; 34(288)24788-98
Epigenetic changes in renal genes dysregulated in mouse and rat models of type 1 diabetes.
Komers R, Mar D, Denisenko O, Xu B, Oyama TT, Bomsztyk K.
Lab Invest. 2013 May; 5(93)543-52
Rubinow KB, Wall VZ, Nelson J, Mar D, Bomsztyk K, Askari B, Lai MA, Smith KD, Han MS, Vivekanandan-Giri A, Pennathur S, Albert CJ, Ford DA, Davis RJ, Bornfeldt KE.
J Biol Chem. 2013 Apr 5; 14(288)9957-70
van Rensburg R, Beyer I, Yao XY, Wang H, Denisenko O, Li ZY, Russell DW, Miller DG, Gregory P, Holmes M, Bomsztyk K, Lieber A.
Gene Ther. 2013 Feb; 2(20)201-14
Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays.
Yu J, Feng Q, Ruan Y, Komers R, Kiviat N, Bomsztyk K.
BMC Mol Biol. 2011 Nov 18; (12)49
Allele-specific transcriptional elongation regulates monoallelic expression of the IGF2BP1 gene.
Thomas BJ, Rubio ED, Krumm N, Broin PO, Bomsztyk K, Welcsh P, Greally JM, Golden AA, Krumm A.
Epigenetics Chromatin. 2011 Aug 3; (4)14
Denisenko O, Lin B, Louey S, Thornburg K, Bomsztyk K, Bagby S.
J Dev Orig Health Dis. 2011 Apr; 2(2)124-33
Mikula M, Bomsztyk K.
J Biol Chem. 2011 Mar 18; 11(286)9763-75
Profiling RNA polymerase II using the fast chromatin immunoprecipitation method.
Nelson J, Denisenko O, Bomsztyk K.
Methods Mol Biol. 2011; (703)219-34
Direct recruitment of insulin receptor and ERK signaling cascade to insulin-inducible gene loci.
Nelson JD, LeBoeuf RC, Bomsztyk K.
Diabetes. 2011 Jan; 1(60)127-37
Aker M, Bomsztyk K, Emery DW.
J Biol Chem. 2010 Nov 26; 48(285)37589-97
Halogenated imidazole derivatives block RNA polymerase II elongation along mitogen inducible genes.
Mikula M, Hanusek K, Paziewska A, Dzwonek A, Rubel T, Bomsztyk K, Ostrowski J.
BMC Mol Biol. 2010 Jan 15; (11)4
The fast chromatin immunoprecipitation method.
Nelson J, Denisenko O, Bomsztyk K.
Methods Mol Biol. 2009; (567)45-57
BRG1 increases transcription of proinflammatory genes in renal ischemia.
Naito M, Zager RA, Bomsztyk K.
J Am Soc Nephrol. 2009 Aug; 8(20)1787-96
Naito M, Shenoy A, Aoyama I, Koopmeiners JS, Komers R, Schnaper HW, Bomsztyk K.
Am J Nephrol. 2009; 2(30)99-111
Naito M, Bomsztyk K, Zager RA.
Am J Pathol. 2009 Jan; 1(174)54-62
Growth and development alter susceptibility to acute renal injury.
Zager RA, Johnson AC, Naito M, Lund SR, Kim N, Bomsztyk K.
Kidney Int. 2008 Sep; 5(74)674-8
Endotoxin mediates recruitment of RNA polymerase II to target genes in acute renal failure.
Naito M, Bomsztyk K, Zager RA.
J Am Soc Nephrol. 2008 Jul; 7(19)1321-30
Flanagin S, Nelson JD, Castner DG, Denisenko O, Bomsztyk K.
Nucleic Acids Res. 2008 Feb; 3(36)e17
Nelson JD, Flanagin S, Kawata Y, Denisenko O, Bomsztyk K.
Am J Physiol Renal Physiol. 2008 Mar; 3(294)F525-33
Sova P, Wang H, Bomsztyk K, Stamatoyannopoulos G, Lieber A.
Hum Gene Ther. 2008 Feb; 2(19)153-66
Denisenko O, Bomsztyk K.
J Mol Biol. 2008 Jan 25; 4(375)1178-87
Zager RA, Johnson AC, Naito M, Bomsztyk K.
Am J Physiol Renal Physiol. 2008 Jan; 1(294)F187-97
Quantitative model for binary measurements of protein-protein interactions.
Shi YY, Miller GA, Denisenko O, Qian H, Bomsztyk K.
J Comput Biol. 2007 Sep; 7(14)1011-23
Clustering coefficients of protein-protein interaction networks.
Miller GA, Shi YY, Qian H, Bomsztyk K.
Phys Rev E Stat Nonlin Soft Matter Phys. 2007 May; 5 Pt 1(75)051910
Feliers D, Lee MJ, Ghosh-Choudhury G, Bomsztyk K, Kasinath BS.
Am J Physiol Renal Physiol. 2007 Aug; 2(293)F607-15
Protocol for the fast chromatin immunoprecipitation (ChIP) method.
Nelson JD, Denisenko O, Bomsztyk K.
Nat Protoc. 2006; 1(1)179-85
Shi YY, Miller GA, Qian H, Bomsztyk K.
Proc Natl Acad Sci U S A. 2006 Aug 1; 31(103)11527-32
Okano K, Schnaper HW, Bomsztyk K, Hayashida T.
J Biol Chem. 2006 Sep 8; 36(281)26196-204
Heterogeneous nuclear ribonucleoprotein K modulates angiotensinogen gene expression in kidney cells.
Wei CC, Zhang SL, Chen YW, Guo DF, Ingelfinger JR, Bomsztyk K, Chan JS.
J Biol Chem. 2006 Sep 1; 35(281)25344-55
Landscape of the hnRNP K protein-protein interactome.
Mikula M, Dzwonek A, Karczmarski J, Rubel T, Dadlez M, Wyrwicz LS, Bomsztyk K, Ostrowski J.
Proteomics. 2006 Apr; 8(6)2395-406
Casein kinases phosphorylate multiple residues spanning the entire hnRNP K length.
Mikula M, Karczmarski J, Dzwonek A, Rubel T, Hennig E, Dadlez M, Bujnicki JM, Bomsztyk K, Ostrowski J.
Biochim Biophys Acta. 2006 Feb; 2(1764)299-306
Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa.
Klimek-Tomczak K, Mikula M, Dzwonek A, Paziewska A, Karczmarski J, Hennig E, Bujnicki JM, Bragoszewski P, Denisenko O, Bomsztyk K, Ostrowski J.
Br J Cancer. 2006 Feb 27; 4(94)586-92
Fast chromatin immunoprecipitation assay.
Nelson JD, Denisenko O, Sova P, Bomsztyk K.
Nucleic Acids Res. 2006 Jan 5; 1(34)e2
Cooperative binding of the hnRNP K three KH domains to mRNA targets.
Paziewska A, Wyrwicz LS, Bujnicki JM, Bomsztyk K, Ostrowski J.
FEBS Lett. 2004 Nov 5; 1-2(577)134-40
Ostrowski J, Klimek-Tomczak K, Wyrwicz LS, Mikula M, Schullery DS, Bomsztyk K.
J Biol Chem. 2004 Dec 24; 52(279)54599-609
Characterization of hnRNP K protein-RNA interactions.
Klimek-Tomczak K, Wyrwicz LS, Jain S, Bomsztyk K, Ostrowski J.
J Mol Biol. 2004 Sep 24; 4(342)1131-41
hnRNP K: one protein multiple processes.
Bomsztyk K, Denisenko O, Ostrowski J.
Bioessays. 2004 Jun; 6(26)629-38
Nuclear shift of hnRNP K protein in neoplasms and other states of enhanced cell proliferation.
Ostrowski J, Bomsztyk K.
Br J Cancer. 2003 Oct 20; 8(89)1493-501
Evans JR, Mitchell SA, Spriggs KA, Ostrowski J, Bomsztyk K, Ostarek D, Willis AE.
Oncogene. 2003 Sep 11; 39(22)8012-20
Transient recruitment of the hnRNP K protein to inducibly transcribed gene loci.
Ostrowski J, Kawata Y, Schullery DS, Denisenko ON, Bomsztyk K.
Nucleic Acids Res. 2003 Jul 15; 14(31)3954-62
Ostareck-Lederer A, Ostareck DH, Cans C, Neubauer G, Bomsztyk K, Superti-Furga G, Hentze MW.
Mol Cell Biol. 2002 Jul; 13(22)4535-43
Higaki Y, Schullery D, Kawata Y, Shnyreva M, Abrass C, Bomsztyk K.
Nucleic Acids Res. 2002 Jun 1; 11(30)2270-9
Kawata Y, Suzuki H, Higaki Y, Denisenko O, Schullery D, Abrass C, Bomsztyk K.
J Biol Chem. 2002 Mar 29; 13(277)11375-84
Ostrowski J, Wyrwicz L, Rychlewski L, Bomsztyk K.
J Biol Chem. 2002 Feb 22; 8(277)6303-10
Denisenko O, Bomsztyk K.
Mol Cell Biol. 2002 Jan; 1(22)286-97
Insulin alters heterogeneous nuclear ribonucleoprotein K protein binding to DNA and RNA.
Ostrowski J, Kawata Y, Schullery DS, Denisenko ON, Higaki Y, Abrass CK, Bomsztyk K.
Proc Natl Acad Sci U S A. 2001 Jul 31; 16(98)9044-9
Ostrowski J, Woszczyński M, Kowalczyk P, Trzeciak L, Hennig E, Bomsztyk K.
J Hepatol. 2000 Jun; 6(32)965-74
Shnyreva M, Schullery DS, Suzuki H, Higaki Y, Bomsztyk K.
J Biol Chem. 2000 May 19; 20(275)15498-503
Ostrowski J, Woszczynski M, Kowalczyk P, Wocial T, Hennig E, Trzeciak L, Janik P, Bomsztyk K.
Br J Cancer. 2000 Mar; 5(82)1041-50
Ostrowski J, Schullery DS, Denisenko ON, Higaki Y, Watts J, Aebersold R, Stempka L, Gschwendt M, Bomsztyk K.
J Biol Chem. 2000 Feb 4; 5(275)3619-28
Schullery DS, Ostrowski J, Denisenko ON, Stempka L, Shnyreva M, Suzuki H, Gschwendt M, Bomsztyk K.
J Biol Chem. 1999 May 21; 21(274)15101-9
Ostrowski J, Trzeciak L, Kołodziejski J, Bomsztyk K.
Br J Cancer. 1998 Nov; 10(78)1301-6
Suzuki H, Denisenko ON, Suzuki Y, Schullery DS, Bomsztyk K.
Am J Physiol. 1998 Oct; 4(275)F518-26
Point mutations in the WD40 domain of Eed block its interaction with Ezh2.
Denisenko O, Shnyreva M, Suzuki H, Bomsztyk K.
Mol Cell Biol. 1998 Oct; 10(18)5634-42
Ostrowski J, Florio SK, Denis GV, Suzuki H, Bomsztyk K.
Oncogene. 1998 Mar 5; 9(16)1223-7
O'Neill BC, Suzuki H, Loomis WP, Denisenko O, Bomsztyk K.
Am J Physiol. 1997 Sep; 3 Pt 2(273)F411-20
Denisenko ON, Bomsztyk K.
Mol Cell Biol. 1997 Aug; 8(17)4707-17
Diverse molecular interactions of the hnRNP K protein.
Bomsztyk K, Van Seuningen I, Suzuki H, Denisenko O, Ostrowski J.
FEBS Lett. 1997 Feb 17; 2(403)113-5
Cloning of a new human gene with short consensus repeats using the EST database.
Nangaku M, Shankland SJ, Kurokawa K, Bomsztyk K, Johnson RJ, Couser WG.
Immunogenetics. 1997; 2(46)99-103
Denisenko ON, O'Neill B, Ostrowski J, Van Seuningen I, Bomsztyk K.
J Biol Chem. 1996 Nov 1; 44(271)27701-6
Suzuki H, O'Neill BC, Suzuki Y, Denisenko ON, Bomsztyk K.
J Biol Chem. 1996 Aug 2; 31(271)18981-8
Valentine MA, Czernik AJ, Rachie N, Hidaka H, Fisher CL, Cambier JC, Bomsztyk K.
J Exp Med. 1995 Dec 1; 6(182)1943-9
Van Seuningen I, Ostrowski J, Bustelo XR, Sleath PR, Bomsztyk K.
J Biol Chem. 1995 Nov 10; 45(270)26976-85
Van Seuningen I, Ostrowski J, Bomsztyk K.
Biochemistry. 1995 Apr 25; 16(34)5644-50
Lawrence O, Rachie N, Qureshi N, Bomsztyk K, Sibley CH.
Infect Immun. 1995 Mar; 3(63)1040-6
Richardson CA, Gordon KL, Couser WG, Bomsztyk K.
Am J Physiol. 1995 Feb; 2 Pt 2(268)F273-8
Qwarnström EE, Ostberg CO, Turk GL, Richardson CA, Bomsztyk K.
J Biol Chem. 1994 Dec 9; 49(269)30765-8
Ostrowski J, Van Seuningen I, Seger R, Rauch CT, Sleath PR, McMullen BA, Bomsztyk K.
J Biol Chem. 1994 Jul 1; 26(269)17626-34
Identification of an inducible 85-kDa nuclear protein kinase.
Rachie NA, Seger R, Valentine MA, Ostrowski J, Bomsztyk K.
J Biol Chem. 1993 Oct 15; 29(268)22143-9
Comparison of IL-1 alpha effectiveness in activating murine pre-B and T cell lines.
Iwasaki T, Sims JE, Grabstein K, Dower SK, Rachie N, Bomsztyk K.
Cytokine. 1993 Sep; 5(5)416-26
Purification of DNA-binding proteins using tandem DNA-affinity column.
Ostrowski J, Bomsztyk K.
Nucleic Acids Res. 1993 Feb 25; 4(21)1045-6
Valentine MA, Bursten SL, Harris WE, Draves KE, Pollok BA, Ostrowski J, Bomsztyk K, Clark EA.
Cell Immunol. 1992 May; 2(141)373-87
Effects of amiloride on distal renal tubule sodium and calcium absorption: dependence on luminal pH.
Mori Y, Machida T, Miyakawa S, Bomsztyk K.
Pharmacol Toxicol. 1992 Mar; 3(70)201-4
Herbimycin A blocks IL-1-induced NF-kappa B DNA-binding activity in lymphoid cell lines.
Iwasaki T, Uehara Y, Graves L, Rachie N, Bomsztyk K.
FEBS Lett. 1992 Feb 24; 2-3(298)240-4
Bursten SL, Harris WE, Bomsztyk K, Lovett D.
J Biol Chem. 1991 Nov 5; 31(266)20732-43
Activation of endothelial-leukocyte adhesion molecule 1 (ELAM-1) gene transcription.
Montgomery KF, Osborn L, Hession C, Tizard R, Goff D, Vassallo C, Tarr PI, Bomsztyk K, Lobb R, Harlan JM.
Proc Natl Acad Sci U S A. 1991 Aug 1; 15(88)6523-7
Ostrowski J, Sims JE, Sibley CH, Valentine MA, Dower SK, Meier KE, Bomsztyk K.
J Biol Chem. 1991 Jul 5; 19(266)12722-33
Bomsztyk K, Rooney JW, Iwasaki T, Rachie NA, Dower SK, Sibley CH.
Cell Regul. 1991 Apr; 4(2)329-35
Huynh TT, Bomsztyk K.
Magnes Trace Elem. 1991-1992; 5-6(10)321-6
Ostrowski J, Sibley CH, Stepinski J, Stanton TH, Smith LL, Bomsztyk K.
Cell Immunol. 1990 Oct 1; 1(130)11-21
Role of cAMP in interleukin-1-induced kappa light chain gene expression in murine B cell line.
Bomsztyk K, Toivola B, Emery DW, Rooney JW, Dower SK, Rachie NA, Sibley CH.
J Biol Chem. 1990 Jun 5; 16(265)9413-7
Molecular heterogeneity of interleukin-1 receptors.
Dower SK, Sims JE, Stanton TH, Slack J, McMahan CJ, Valentine MA, Bomsztyk K.
Ann N Y Acad Sci. 1990; (594)231-9
Evidence for different interleukin 1 receptors in murine B- and T-cell lines.
Bomsztyk K, Sims JE, Stanton TH, Slack J, McMahan CJ, Valentine MA, Dower SK.
Proc Natl Acad Sci U S A. 1989 Oct; 20(86)8034-8
Properties of interleukin-1 and interferon-gamma receptors in B lymphoid cell line.
Bomsztyk K, Stanton TH, Smith LL, Rachie NA, Dower SK.
J Biol Chem. 1989 Apr 15; 11(264)6052-7
Interaction of signal transduction pathways in mediating acid secretion by rat parietal cells.
Ostrowski J, Bomsztyk K.
Am J Physiol. 1989 Apr; 4 Pt 1(256)C873-9
Ostrowski J, Meier KE, Stanton TH, Smith LL, Bomsztyk K.
J Biol Chem. 1988 Sep 25; 27(263)13786-90
Smith LL, Stanton TH, Calalb MB, Bomsztyk K.
J Biol Chem. 1988 May 25; 15(263)7359-63
Bicarbonate absorption stimulates active calcium absorption in the rat proximal tubule.
Bomsztyk K, Calalb MB.
J Clin Invest. 1988 May; 5(81)1455-61
A microelectrometric titration method for measurement of total intracellular Cl- concentration.
Bomsztyk K, Calalb MB, Smith L, Stanton TH.
Am J Physiol. 1988 Jan; 1 Pt 1(254)C200-5
Calalb MB, Stanton TH, Smith L, Cragoe EJ Jr, Bomsztyk K.
J Biol Chem. 1987 Mar 15; 8(262)3680-4
HCO3 accumulation in proximal tubule: roles of carbonic anhydrase, luminal buffers, and pH.
Bomsztyk K, Swenson ER, Calalb MB.
Am J Physiol. 1987 Mar; 3 Pt 2(252)F501-8
A new microelectrode method for simultaneous measurement of pH and PCO2.
Bomsztyk K, Calalb MB.
Am J Physiol. 1986 Nov; 5 Pt 2(251)F933-7
Chloride transport by rat renal proximal tubule: effects of bicarbonate absorption.
Bomsztyk K
Am J Physiol. 1986 Jun; 6 Pt 2(250)F1046-54
Stanton TH, Maynard M, Bomsztyk K.
J Biol Chem. 1986 May 5; 13(261)5699-701
Dependence of ion fluxes on fluid transport by rat proximal tubule.
Bomsztyk K, Wright FS.
Am J Physiol. 1986 Apr; 4 Pt 2(250)F680-9
Calcium transport by the proximal tubule.
Wright FS, Bomsztyk K.
Adv Exp Med Biol. 1986; (208)165-70
Effects of luminal fluid anions on calcium transport by proximal tubule.
Bomsztyk K, George JP, Wright FS.
Am J Physiol. 1984 May; 5 Pt 2(246)F600-8
Research Summary
Detrimental epigenetic processes are emerging as major pathophysiologic culprits of disease including cancer, inflammation, infection and organ injury. These processes involve signaling, transcription and epigenetic factors that regulate gene transcription in the chromatin environment, or the epigenome. To better define epigenetic processes in health and disease, our laboratory has been developing high throughput and sensitivity epigenetic platforms and computational tools and have applied them to study acute organ injury, cancer and inflammation.