faculty

Dustin Maly

djmaly@uw.edu

University of Washington,

Biophysical and Structural Biology

Cell Signaling & Cell/Environment Interactions

Chemical Biology of Signal Transduction

Faculty Contact Information

Building: CHB Room: 404D Box: 351700 Phone: 206-543-1653

Lab Information

Box: 351700 http://depts.washington.edu/malylab/wordpress

Accepting Students For:

Rotation, Autumn

Rotation, Spring

Rotation, Winter

Permanent

Publications

The following publications were retrieved from PubMed:

Chemoproteomic Method for Profiling Inhibitor-Bound Kinase Complexes.

Fang L, Chakraborty S, Dieter EM, Potter ZE, Lombard CK, Maly DJ.

J Am Chem Soc. 2019 Jul 5;

P-glycoprotein mediated efflux reduces the in vivo efficacy of a therapeutic targeting the gastrointestinal parasite Cryptosporidium.

Arnold SLM, Choi R, Hulverson MA, Whitman GR, McCloskey MC, Dorr CS, Vidadala RSR, Khatod M, Morada M, Barrett LK, Maly DJ, Yarlett N, Van Voorhis WC.

J Infect Dis. 2019 Jun 8;

Targeting Dynamic ATP-Binding Site Features Allows Discrimination between Highly Homologous Protein Kinases.

Chakraborty S, Inukai T, Fang L, Golkowski M, Maly DJ.

ACS Chem Biol. 2019 Jun 21; 6(14)1249-1259

Bumped kinase inhibitor 1369 is effective against Cystoisospora suis in vivo and in vitro.

Shrestha A, Ojo KK, Koston F, Ruttkowski B, Vidadala RSR, Dorr CS, Navaluna ED, Whitman GR, Barrett KF, Barrett LK, Hulverson MA, Choi R, Michaels SA, Maly DJ, Hemphill A, Van Voorhis WC, Joachim A.

Int J Parasitol Drugs Drug Resist. 2019 Apr 2; (10)9-19

A Combined Approach Reveals a Regulatory Mechanism Coupling Src's Kinase Activity, Localization, and Phosphotransferase-Independent Functions.

Ahler E, Register AC, Chakraborty S, Fang L, Dieter EM, Sitko KA, Vidadala RSR, Trevillian BM, Golkowski M, Gelman H, Stephany JJ, Rubin AF, Merritt EA, Fowler DM, Maly DJ.

Mol Cell. 2019 Apr 18; 2(74)393-408.e20

A Chemically Disrupted Proximity System for Controlling Dynamic Cellular Processes.

Cunningham-Bryant D, Dieter EM, Foight GW, Rose JC, Loutey DE, Maly DJ.

J Am Chem Soc. 2019 Feb 27; 8(141)3352-3355

Small molecule inhibition of IRE1α kinase/RNase has anti-fibrotic effects in the lung.

Thamsen M, Ghosh R, Auyeung VC, Brumwell A, Chapman HA, Backes BJ, Perara G, Maly DJ, Sheppard D, Papa FR.

PLoS One. 2019; 1(14)e0209824

Pharmacokinetics and In Vivo Efficacy of Pyrazolopyrimidine, Pyrrolopyrimidine, and 5-Aminopyrazole-4-Carboxamide Bumped Kinase Inhibitors against Toxoplasmosis.

Hulverson MA, Bruzual I, McConnell EV, Huang W, Vidadala RSR, Choi R, Arnold SLM, Whitman GR, McCloskey MC, Barrett LK, Rivas KL, Scheele S, DeRocher AE, Parsons M, Ojo KK, Maly DJ, Fan E, Van Voorhis WC, Doggett JS.

J Infect Dis. 2019 Apr 16; 9(219)1464-1473

Allosteric Modulation of JNK Docking Site Interactions with ATP-Competitive Inhibitors.

Lombard CK, Davis AL, Inukai T, Maly DJ.

Biochemistry. 2018 Oct 9; 40(57)5897-5909

Kinome chemoproteomics characterization of pyrrolo[3,4-c]pyrazoles as potent and selective inhibitors of glycogen synthase kinase 3.

Golkowski M, Perera GK, Vidadala VN, Ojo KK, Van Voorhis WC, Maly DJ, Ong SE.

Mol Omics. 2018 Feb 12; 1(14)26-36

"Examining RAS pathway rewiring with a chemically inducible activator of RAS".

Rose JC, Dieter EM, Cunningham-Bryant D, Maly DJ.

Small GTPases. 2018 Apr 10; 1-8

Toxoplasma Calcium-Dependent Protein Kinase 1 Inhibitors: Probing Activity and Resistance Using Cellular Thermal Shift Assays.

Scheele S, Geiger JA, DeRocher AE, Choi R, Smith TR, Hulverson MA, Vidadala RSR, Barrett LK, Maly DJ, Merritt EA, Ojo KK, Van Voorhis WC, Parsons M.

Antimicrob Agents Chemother. 2018 Jun; 6(62)

7 H-Pyrrolo[2,3- d]pyrimidin-4-amine-Based Inhibitors of Calcium-Dependent Protein Kinase 1 Have Distinct Inhibitory and Oral Pharmacokinetic Characteristics Compared with 1 H-Pyrazolo[3,4- d]pyrimidin-4-amine-Based Inhibitors.

Vidadala RSR, Golkowski M, Hulverson MA, Choi R, McCloskey MC, Whitman GR, Huang W, Arnold SLM, Barrett LK, Fan E, Merritt EA, Van Voorhis WC, Ojo KK, Maly DJ.

ACS Infect Dis. 2018 Apr 13; 4(4)516-522

In vitro growth inhibition of Theileria equi by bumped kinase inhibitors.

Gimenez F, Hines SA, Evanoff R, Ojo KK, Van Voorhis WC, Maly DJ, Vidadala RSR, Mealey RH.

Vet Parasitol. 2018 Feb 15; (251)90-94

In vitro efficacy of bumped kinase inhibitors against Besnoitia besnoiti tachyzoites.

Jiménez-Meléndez A, Ojo KK, Wallace AM, Smith TR, Hemphill A, Balmer V, Regidor-Cerrillo J, Ortega-Mora LM, Hehl AB, Fan E, Maly DJ, Van Voorhis WC, Álvarez-García G.

Int J Parasitol. 2017 Oct; 12(47)811-821

Advances in bumped kinase inhibitors for human and animal therapy for cryptosporidiosis.

Hulverson MA, Choi R, Arnold SLM, Schaefer DA, Hemphill A, McCloskey MC, Betzer DP, Müller J, Vidadala RSR, Whitman GR, Rivas KL, Barrett LK, Hackman RC, Love MS, McNamara CW, Shaughnessy TK, Kondratiuk A, Kurnick M, Banfor PN, Lynch JJ, Freiberg GM, Kempf DJ, Maly DJ, Riggs MW, Ojo KK, Van Voorhis WC.

Int J Parasitol. 2017 Oct; 12(47)753-763

Rheostatic Control of Cas9-Mediated DNA Double Strand Break (DSB) Generation and Genome Editing.

Rose JC, Stephany JJ, Wei CT, Fowler DM, Maly DJ.

ACS Chem Biol. 2018 Feb 16; 2(13)438-442

Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics.

Rose JC, Stephany JJ, Valente WJ, Trevillian BM, Dang HV, Bielas JH, Maly DJ, Fowler DM.

Nat Methods. 2017 Sep; 9(14)891-896

Proteomic Profiling of Protein Kinase Inhibitor Targets by Mass Spectrometry.

Golkowski M, Maly DJ, Ong SE.

Methods Mol Biol. 2017; (1636)105-117

Allosteric Modulation of Src Family Kinases with ATP-Competitive Inhibitors.

Methods Mol Biol. 2017; (1636)79-89

Necessity of Bumped Kinase Inhibitor Gastrointestinal Exposure in Treating Cryptosporidium Infection.

Arnold SLM, Choi R, Hulverson MA, Schaefer DA, Vinayak S, Vidadala RSR, McCloskey MC, Whitman GR, Huang W, Barrett LK, Ojo KK, Fan E, Maly DJ, Riggs MW, Striepen B, Van Voorhis WC.

J Infect Dis. 2017 Jul 1; 1(216)55-63

5-Aminopyrazole-4-Carboxamide-Based Compounds Prevent the Growth of Cryptosporidium parvum.

Huang W, Choi R, Hulverson MA, Zhang Z, McCloskey MC, Schaefer DA, Whitman GR, Barrett LK, Vidadala RSR, Riggs MW, Maly DJ, Van Voorhis WC, Ojo KK, Fan E.

Antimicrob Agents Chemother. 2017 Aug; 8(61)

Development of a murine vertical transmission model for Toxoplasma gondii oocyst infection and studies on the efficacy of bumped kinase inhibitor (BKI)-1294 and the naphthoquinone buparvaquone against congenital toxoplasmosis.

Müller J, Aguado-Martínez A, Ortega-Mora LM, Moreno-Gonzalo J, Ferre I, Hulverson MA, Choi R, McCloskey MC, Barrett LK, Maly DJ, Ojo KK, Van Voorhis W, Hemphill A.

J Antimicrob Chemother. 2017 Aug 1; 8(72)2334-2341

Targeting ABL-IRE1α Signaling Spares ER-Stressed Pancreatic β Cells to Reverse Autoimmune Diabetes.

Morita S, Villalta SA, Feldman HC, Register AC, Rosenthal W, Hoffmann-Petersen IT, Mehdizadeh M, Ghosh R, Wang L, Colon-Negron K, Meza-Acevedo R, Backes BJ, Maly DJ, Bluestone JA, Papa FR.

Cell Metab. 2017 May 2; 5(25)1207

Targeting ABL-IRE1α Signaling Spares ER-Stressed Pancreatic β Cells to Reverse Autoimmune Diabetes.

Morita S, Villalta SA, Feldman HC, Register AC, Rosenthal W, Hoffmann-Petersen IT, Mehdizadeh M, Ghosh R, Wang L, Colon-Negron K, Meza-Acevedo R, Backes BJ, Maly DJ, Bluestone JA, Papa FR.

Cell Metab. 2017 Apr 4; 4(25)883-897.e8

Bumped-Kinase Inhibitors for Cryptosporidiosis Therapy.

Hulverson MA, Vinayak S, Choi R, Schaefer DA, Castellanos-Gonzalez A, Vidadala RSR, Brooks CF, Herbert GT, Betzer DP, Whitman GR, Sparks HN, Arnold SLM, Rivas KL, Barrett LK, White AC Jr, Maly DJ, Riggs MW, Striepen B, Van Voorhis WC, Ojo KK.

J Infect Dis. 2017 Apr 15; 8(215)1275-1284

Two Novel Calcium-Dependent Protein Kinase 1 Inhibitors Interfere with Vertical Transmission in Mice Infected with Neospora caninum Tachyzoites.

Müller J, Aguado-Martínez A, Balmer V, Maly DJ, Fan E, Ortega-Mora LM, Ojo KK, Van Voorhis WC, Hemphill A.

Antimicrob Agents Chemother. 2017 Apr; 4(61)

Kinobead and Single-Shot LC-MS Profiling Identifies Selective PKD Inhibitors.

Golkowski M, Vidadala RS, Lombard CK, Suh HW, Maly DJ, Ong SE.

J Proteome Res. 2017 Mar 3; 3(16)1216-1227

Extended-spectrum antiprotozoal bumped kinase inhibitors: A review.

Van Voorhis WC, Doggett JS, Parsons M, Hulverson MA, Choi R, Arnold SLM, Riggs MW, Hemphill A, Howe DK, Mealey RH, Lau AOT, Merritt EA, Maly DJ, Fan E, Ojo KK.

Exp Parasitol. 2017 Sep; (180)71-83

Novel Bumped Kinase Inhibitors Are Safe and Effective Therapeutics in the Calf Clinical Model for Cryptosporidiosis.

Schaefer DA, Betzer DP, Smith KD, Millman ZG, Michalski HC, Menchaca SE, Zambriski JA, Ojo KK, Hulverson MA, Arnold SL, Rivas KL, Vidadala RS, Huang W, Barrett LK, Maly DJ, Fan E, Van Voorhis WC, Riggs MW.

J Infect Dis. 2016 Dec 15; 12(214)1856-1864

Reduced Activity of Mutant Calcium-Dependent Protein Kinase 1 Is Compensated in Plasmodium falciparum through the Action of Protein Kinase G.

Bansal A, Ojo KK, Mu J, Maly DJ, Van Voorhis WC, Miller LH.

MBio. 2016 Dec 6; 6(7)

A computationally engineered RAS rheostat reveals RAS-ERK signaling dynamics.

Rose JC, Huang PS, Camp ND, Ye J, Leidal AM, Goreshnik I, Trevillian BM, Dickinson MS, Cunningham-Bryant D, Debnath J, Baker D, Wolf-Yadlin A, Maly DJ.

Nat Chem Biol. 2017 Jan; 1(13)119-126

Profiling the Dual Enzymatic Activities of the Serine/Threonine Kinase IRE1α.

Feldman HC, Maly DJ.

Methods Mol Biol. 2017; (1513)233-242

5-Aminopyrazole-4-carboxamide analogues are selective inhibitors of Plasmodium falciparum microgametocyte exflagellation and potential malaria transmission blocking agents.

Huang W, Hulverson MA, Zhang Z, Choi R, Hart KJ, Kennedy M, Vidadala RSR, Maly DJ, Van Voorhis WC, Lindner SE, Fan E, Ojo KK.

Bioorg Med Chem Lett. 2016 Nov 15; 22(26)5487-5491

Selective inhibition of Sarcocystis neurona calcium-dependent protein kinase 1 for equine protozoal myeloencephalitis therapy.

Ojo KK, Dangoudoubiyam S, Verma SK, Scheele S, DeRocher AE, Yeargan M, Choi R, Smith TR, Rivas KL, Hulverson MA, Barrett LK, Fan E, Maly DJ, Parsons M, Dubey JP, Howe DK, Van Voorhis WC.

Int J Parasitol. 2016 Dec; 13-14(46)871-880

Development of an Orally Available and Central Nervous System (CNS) Penetrant Toxoplasma gondii Calcium-Dependent Protein Kinase 1 (TgCDPK1) Inhibitor with Minimal Human Ether-a-go-go-Related Gene (hERG) Activity for the Treatment of Toxoplasmosis.

Vidadala RS, Rivas KL, Ojo KK, Hulverson MA, Zambriski JA, Bruzual I, Schultz TL, Huang W, Zhang Z, Scheele S, DeRocher AE, Choi R, Barrett LK, Siddaramaiah LK, Hol WG, Fan E, Merritt EA, Parsons M, Freiberg G, Marsh K, Kempf DJ, Carruthers VB, Isoherranen N, Doggett JS, Van Voorhis WC, Maly DJ.

J Med Chem. 2016 Jul 14; 13(59)6531-46

Structural and Functional Analysis of the Allosteric Inhibition of IRE1α with ATP-Competitive Ligands.

Feldman HC, Tong M, Wang L, Meza-Acevedo R, Gobillot TA, Lebedev I, Gliedt MJ, Hari SB, Mitra AK, Backes BJ, Papa FR, Seeliger MA, Maly DJ.

ACS Chem Biol. 2016 Aug 19; 8(11)2195-205

A novel protein kinase is essential in bloodstream Trypanosoma brucei.

Jensen BC, Booster N, Vidadala RS, Maly DJ, Parsons M.

Int J Parasitol. 2016 Jul; 8(46)479-83

Biochemical Screening of Five Protein Kinases from Plasmodium falciparum against 14,000 Cell-Active Compounds.

Crowther GJ, Hillesland HK, Keyloun KR, Reid MC, Lafuente-Monasterio MJ, Ghidelli-Disse S, Leonard SE, He P, Jones JC, Krahn MM, Mo JS, Dasari KS, Fox AM, Boesche M, El Bakkouri M, Rivas KL, Leroy D, Hui R, Drewes G, Maly DJ, Van Voorhis WC, Ojo KK.

PLoS One. 2016; 3(11)e0149996

Bumped kinase inhibitor prohibits egression in Babesia bovis.

Pedroni MJ, Vidadala RS, Choi R, Keyloun KR, Reid MC, Murphy RC, Barrett LK, Van Voorhis WC, Maly DJ, Ojo KK, Lau AO.

Vet Parasitol. 2016 Jan 15; (215)22-8

SAR Studies of 5-Aminopyrazole-4-carboxamide Analogues as Potent and Selective Inhibitors of Toxoplasma gondii CDPK1.

Huang W, Ojo KK, Zhang Z, Rivas K, Vidadala RS, Scheele S, DeRocher AE, Choi R, Hulverson MA, Barrett LK, Bruzual I, Siddaramaiah LK, Kerchner KM, Kurnick MD, Freiberg GM, Kempf D, Hol WG, Merritt EA, Neckermann G, de Hostos EL, Isoherranen N, Maly DJ, Parsons M, Doggett JS, Van Voorhis WC, Fan E.

ACS Med Chem Lett. 2015 Dec 10; 12(6)1184-1189

Conversion of a Single Polypharmacological Agent into Selective Bivalent Inhibitors of Intracellular Kinase Activity.

Gower CM, Thomas JR, Harrington E, Murphy J, Chang ME, Cornella-Taracido I, Jain RK, Schirle M, Maly DJ.

ACS Chem Biol. 2016 Jan 15; 1(11)121-31

In Vitro and In Vivo Effects of the Bumped Kinase Inhibitor 1294 in the Related Cyst-Forming Apicomplexans Toxoplasma gondii and Neospora caninum.

Winzer P, Müller J, Aguado-Martínez A, Rahman M, Balmer V, Manser V, Ortega-Mora LM, Ojo KK, Fan E, Maly DJ, Van Voorhis WC, Hemphill A.

Antimicrob Agents Chemother. 2015 Oct; 10(59)6361-74

Radiotherapy followed by aurora kinase inhibition targets tumor-propagating cells in human glioblastoma.

Li N, Maly DJ, Chanthery YH, Sirkis DW, Nakamura JL, Berger MS, James CD, Shokat KM, Weiss WA, Persson AI.

Mol Cancer Ther. 2015 Feb; 2(14)419-28

Druggable sensors of the unfolded protein response.

Maly DJ, Papa FR.

Nat Chem Biol. 2014 Nov; 11(10)892-901

SH2-catalytic domain linker heterogeneity influences allosteric coupling across the SFK family.

Biochemistry. 2014 Nov 11; 44(53)6910-23

Allosteric inhibition of the IRE1α RNase preserves cell viability and function during endoplasmic reticulum stress.

Ghosh R, Wang L, Wang ES, Perera BG, Igbaria A, Morita S, Prado K, Thamsen M, Caswell D, Macias H, Weiberth KF, Gliedt MJ, Alavi MV, Hari SB, Mitra AK, Bhhatarai B, Schürer SC, Snapp EL, Gould DB, German MS, Backes BJ, Maly DJ, Oakes SA, Papa FR.

Cell. 2014 Jul 31; 3(158)534-48

Divergent modulation of Src-family kinase regulatory interactions with ATP-competitive inhibitors.

Leonard SE, Register AC, Krishnamurty R, Brighty GJ, Maly DJ.

ACS Chem Biol. 2014 Aug 15; 8(9)1894-905

The gatekeeper residue and beyond: homologous calcium-dependent protein kinases as drug development targets for veterinarian Apicomplexa parasites.

Keyloun KR, Reid MC, Choi R, Song Y, Fox AMW, Hillesland HK, Zhang Z, Vidadala R, Merritt EA, Lau AOT, Maly DJ, Fan E, Barrett LK, Van Voorhis WC, Ojo KK.

Parasitology. 2014 Sep; 11(141)1499-1509

Conformation-selective ATP-competitive inhibitors control regulatory interactions and noncatalytic functions of mitogen-activated protein kinases.

Hari SB, Merritt EA, Maly DJ.

Chem Biol. 2014 May 22; 5(21)628-35

Bumped kinase inhibitor 1294 treats established Toxoplasma gondii infection.

Doggett JS, Ojo KK, Fan E, Maly DJ, Van Voorhis WC.

Antimicrob Agents Chemother. 2014 Jun; 6(58)3547-9

Neospora caninum calcium-dependent protein kinase 1 is an effective drug target for neosporosis therapy.

Ojo KK, Reid MC, Kallur Siddaramaiah L, Müller J, Winzer P, Zhang Z, Keyloun KR, Vidadala RS, Merritt EA, Hol WG, Maly DJ, Fan E, Van Voorhis WC, Hemphill A.

PLoS One. 2014; 3(9)e92929

Rapid profiling of protein kinase inhibitors by quantitative proteomics.

Golkowski M, Brigham JL, Perera GK, Romano GE, Maly DJ, Ong SE.

Medchemcomm. 2014 Mar; 3(5)363-369

Bivalent inhibitors of protein kinases.

Gower CM, Chang ME, Maly DJ.

Crit Rev Biochem Mol Biol. 2014 Mar-Apr; 2(49)102-15

Development of potent and selective Plasmodium falciparum calcium-dependent protein kinase 4 (PfCDPK4) inhibitors that block the transmission of malaria to mosquitoes.

Vidadala RS, Ojo KK, Johnson SM, Zhang Z, Leonard SE, Mitra A, Choi R, Reid MC, Keyloun KR, Fox AM, Kennedy M, Silver-Brace T, Hume JC, Kappe S, Verlinde CL, Fan E, Merritt EA, Van Voorhis WC, Maly DJ.

Eur J Med Chem. 2014 Mar 3; (74)562-73

Potent and selective inhibitors of CDPK1 from T. gondii and C. parvum based on a 5-aminopyrazole-4-carboxamide scaffold.

Zhang Z, Ojo KK, Vidadala R, Huang W, Geiger JA, Scheele S, Choi R, Reid MC, Keyloun KR, Rivas K, Siddaramaiah LK, Comess KM, Robinson KP, Merta PJ, Kifle L, Hol WG, Parsons M, Merritt EA, Maly DJ, Verlinde CL, Van Voorhis WC, Fan E.

ACS Med Chem Lett. 2014 Jan 9; 1(5)40-44

A specific inhibitor of PfCDPK4 blocks malaria transmission: chemical-genetic validation.

Ojo KK, Eastman RT, Vidadala R, Zhang Z, Rivas KL, Choi R, Lutz JD, Reid MC, Fox AM, Hulverson MA, Kennedy M, Isoherranen N, Kim LM, Comess KM, Kempf DJ, Verlinde CL, Su XZ, Kappe SH, Maly DJ, Fan E, Van Voorhis WC.

J Infect Dis. 2014 Jan 15; 2(209)275-84

Conformation-selective inhibitors reveal differences in the activation and phosphate-binding loops of the tyrosine kinases Abl and Src.

Hari SB, Perera BG, Ranjitkar P, Seeliger MA, Maly DJ.

ACS Chem Biol. 2013 Dec 20; 12(8)2734-43

A novel calcium-dependent protein kinase inhibitor as a lead compound for treating cryptosporidiosis.

Castellanos-Gonzalez A, White AC Jr, Ojo KK, Vidadala RS, Zhang Z, Reid MC, Fox AM, Keyloun KR, Rivas K, Irani A, Dann SM, Fan E, Maly DJ, Van Voorhis WC.

J Infect Dis. 2013 Oct 15; 8(208)1342-8

Sequence determinants of a specific inactive protein kinase conformation.

Hari SB, Merritt EA, Maly DJ.

Chem Biol. 2013 Jun 20; 6(20)806-15

Label transfer reagents to probe p38 MAPK binding partners.

Andrews SS, Hill ZB, Perera BG, Maly DJ.

Chembiochem. 2013 Jan 21; 2(14)209-16

A hexylchloride-based catch-and-release system for chemical proteomic applications.

Brigham JL, Perera BG, Maly DJ.

ACS Chem Biol. 2013 Apr 19; 4(8)691-9

Active site profiling reveals coupling between domains in SRC-family kinases.

Krishnamurty R, Brigham JL, Leonard SE, Ranjitkar P, Larson ET, Dale EJ, Merritt EA, Maly DJ.

Nat Chem Biol. 2013 Jan; 1(9)43-50

Affinity-based probes based on type II kinase inhibitors.

Ranjitkar P, Perera BG, Swaney DL, Hari SB, Larson ET, Krishnamurty R, Merritt EA, Villén J, Maly DJ.

J Am Chem Soc. 2012 Nov 21; 46(134)19017-25

Divergent allosteric control of the IRE1α endoribonuclease using kinase inhibitors.

Wang L, Perera BG, Hari SB, Bhhatarai B, Backes BJ, Seeliger MA, Schürer SC, Oakes SA, Papa FR, Maly DJ.

Nat Chem Biol. 2012 Dec; 12(8)982-9

Determination of the kinetics and thermodynamics of ligand binding to a specific inactive conformation in protein kinases.

Hari SB, Ranjitkar P, Maly DJ.

Methods Mol Biol. 2012; (928)153-9

Affinity purification of protein kinases that adopt a specific inactive conformation.

Ranjitkar P, Maly DJ.

Methods Mol Biol. 2012; (928)143-51

Benzoylbenzimidazole-based selective inhibitors targeting Cryptosporidium parvum and Toxoplasma gondii calcium-dependent protein kinase-1.

Zhang Z, Ojo KK, Johnson SM, Larson ET, He P, Geiger JA, Castellanos-Gonzalez A, White AC Jr, Parsons M, Merritt EA, Maly DJ, Verlinde CL, Van Voorhis WC, Fan E.

Bioorg Med Chem Lett. 2012 Aug 15; 16(22)5264-7

Transmission of malaria to mosquitoes blocked by bumped kinase inhibitors.

Ojo KK, Pfander C, Mueller NR, Burstroem C, Larson ET, Bryan CM, Fox AM, Reid MC, Johnson SM, Murphy RC, Kennedy M, Mann H, Leibly DJ, Hewitt SN, Verlinde CL, Kappe S, Merritt EA, Maly DJ, Billker O, Van Voorhis WC.

J Clin Invest. 2012 Jun; 6(122)2301-5

Multiple determinants for selective inhibition of apicomplexan calcium-dependent protein kinase CDPK1.

Larson ET, Ojo KK, Murphy RC, Johnson SM, Zhang Z, Kim JE, Leibly DJ, Fox AM, Reid MC, Dale EJ, Perera BG, Kim J, Hewitt SN, Hol WG, Verlinde CL, Fan E, Van Voorhis WC, Maly DJ, Merritt EA.

J Med Chem. 2012 Mar 22; 6(55)2803-10

Development of Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) inhibitors with potent anti-toxoplasma activity.

Johnson SM, Murphy RC, Geiger JA, DeRocher AE, Zhang Z, Ojo KK, Larson ET, Perera BG, Dale EJ, He P, Reid MC, Fox AM, Mueller NR, Merritt EA, Fan E, Parsons M, Van Voorhis WC, Maly DJ.

J Med Chem. 2012 Mar 8; 5(55)2416-26

Targeting diverse signaling interaction sites allows the rapid generation of bivalent kinase inhibitors.

Hill ZB, Perera BG, Andrews SS, Maly DJ.

ACS Chem Biol. 2012 Mar 16; 3(7)487-95

Biochemical and pharmacological profiling of the pro-survival protein Bcl-xL.

Goreshnik I, Brock AM, Maly DJ.

Bioorg Med Chem Lett. 2011 Sep 1; 17(21)4951-5

Intracellular delivery of bioactive molecules using light-addressable nanocapsules.

Gregersen KA, Hill ZB, Gadd JC, Fujimoto BS, Maly DJ, Chiu DT.

ACS Nano. 2010 Dec 28; 12(4)7603-11

Discovery of Potent and Selective Inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from C. parvum and T. gondii.

Murphy RC, Ojo KK, Larson ET, Castellanos-Gonzalez A, Perera BG, Keyloun KR, Kim JE, Bhandari JG, Muller NR, Verlinde CL, White AC Jr, Merritt EA, Van Voorhis WC, Maly DJ.

ACS Med Chem Lett. 2010 Oct 14; 7(1)331-335

Protein kinase affinity reagents based on a 5-aminoindazole scaffold.

Krishnamurty R, Brock AM, Maly DJ.

Bioorg Med Chem Lett. 2011 Jan 1; 1(21)550-4

Bivalent inhibitors of the tyrosine kinases ABL and SRC: determinants of potency and selectivity.

Hill ZB, Perera BG, Maly DJ.

Mol Biosyst. 2011 Feb; 2(7)447-56

Toxoplasma gondii calcium-dependent protein kinase 1 is a target for selective kinase inhibitors.

Ojo KK, Larson ET, Keyloun KR, Castaneda LJ, Derocher AE, Inampudi KK, Kim JE, Arakaki TL, Murphy RC, Zhang L, Napuli AJ, Maly DJ, Verlinde CL, Buckner FS, Parsons M, Hol WG, Merritt EA, Van Voorhis WC.

Nat Struct Mol Biol. 2010 May; 5(17)602-7

Affinity reagents that target a specific inactive form of protein kinases.

Ranjitkar P, Brock AM, Maly DJ.

Chem Biol. 2010 Feb 26; 2(17)195-206

Biochemical mechanisms of resistance to small-molecule protein kinase inhibitors.

Krishnamurty R, Maly DJ.

ACS Chem Biol. 2010 Jan 15; 1(5)121-38

A small molecule-regulated guanine nucleotide exchange factor.

Goreshnik I, Maly DJ.

J Am Chem Soc. 2010 Jan 27; 3(132)938-40

A chemical genetic method for generating bivalent inhibitors of protein kinases.

Hill ZB, Perera BG, Maly DJ.