faculty

David Shechner

shechner@uw.edu

University of Washington, 

Biophysical and Structural Biology

Developmental Biology, Stem Cells & Aging

Gene Expression, Cell Cycle & Chromosome Biology

Noncoding RNAs and the molecular basis of mammalian nuclear architecture

Faculty Contact Information

Building: Health Sciences Center Room: K 335A Box: 357750 Phone: 206-543-0776 Alt Phone: 617-823-4043 https://depts.washington.edu/phcol/faculty/shechner.php

Lab Information

Location: Health Sciences Center Building: K Wing Room: K 554

Accepting Students For:

Rotation, Autumn
Rotation, Spring
Rotation, Summer
Rotation, Winter
Permanent

Publications

Directed evolution of split APEX peroxidase

Han Y, Martell J, Branon T, Shechner DM, Ellisman M, Ting AY.

ACS Chemical Biology. Forthcoming;

My Bibliography [journal]

High-throughput identification of RNA nuclear enrichment sequences.

Shukla CJ, McCorkindale AL, Gerhardinger C, Korthauer KD, Cabili MN, Shechner DM, Irizarry RA, Maass PG, Rinn JL.

The EMBO journal. 2018; 37(6).

PubMed [journal]
PMID:
29335281
PMCID:
PMC5852646

Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING).

Maass PG, Barutcu AR, Shechner DM, Weiner CL, Melé M, Rinn JL.

Nature structural & molecular biology. 2018; 25(2):176-184. NIHMSID: NIHMS928251

PubMed [journal]
PMID:
29343869
PMCID:
PMC5805655

Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking.

Kaewsapsak P, Shechner DM, Mallard W, Rinn JL, Ting AY.

eLife. 2017; 6.

PubMed [journal]
PMID:
29239719
PMCID:
PMC5730372

Chromatin environment, transcriptional regulation, and splicing distinguish lincRNAs and mRNAs.

Melé M, Mattioli K, Mallard W, Shechner DM, Gerhardinger C, Rinn JL.

Genome research. 2017; 27(1):27-37.

PubMed [journal]
PMID:
27927715
PMCID:
PMC5204342

Research Summary

My laboratory aims to decipher the molecular mechanisms by which mammalian cells establish and utilize higher-order chromatin structures, with an emphasis on the interplay between noncoding RNAs (ncRNAs) and nuclear architecture. Using our suite of newly developed technologies for probing and manipulating ncRNA function in situ—combined with an array of biochemical, genomic, and chemical biological approaches—we aim to elucidate the mechanisms by which ncRNAs influence nuclear organization at all levels. We believe this will enable new routes towards novel biotechnologies and therapeutics.