faculty

Devin Schweppe

dkschwep@uw.edu

Assistant Professor

University of Washington

Cancer Biology

Cell Signaling & Cell/Environment Interactions

Microbiology, Infection & Immunity

Systems proteomics and intelligent mass spectrometry

Faculty Contact Information

Building: Foege Building Room: S-041 Box: Box 355065 Phone: 206-616-6031 http://www.schweppelab.org/

Research Summary

Modern proteomics enables quantitation of up to 10,000 proteins from complex cellular matrices. At the interface of cell biology, biochemistry and proteomics, we are attempting to understand how proteins govern key cellular pathways involved in normal cellular function and disease. We are building adaptive strategies to harness spectral data on the millisecond time scale to determine quantitative disparities between cellular states. Intelligent data acquisition in mass spectrometry driven proteomics can enhance the accuracy and efficiency of proteomics data collection.

DEI Statement

The lab is dedicated to providing a harassment-free workplace for everyone, regardless of gender, gender identity/expression, age, sexual orientation, disability, socioeconomics, physical appearance, body size, race, national origin, or religion. We do not tolerate harassment of lab members in any form. All lab members/visitors are expected to agree to the lab’s code of conduct (lab handbook). We do not tolerate the perpetuation of stereotypes; we do not tolerate other acts of microaggression. We are a team. We stand up for one another. We learn from each other. We hold each other accountable.

Training Summary

As a member of the Genome Science faculty, I have engaged in DEI training with both the Lavender Rights Project and Greg Taylor. These trainings were focused in part on addressing the effects of racism, implicit biases, and micro/macroaggressions. As a mentor, I participated in the New Faculty Transition Program to learn how to build upon skills to run a successful research program. This program included mentoring from leaders in the Seattle research community.

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Publications

The following publications were retrieved from PubMed:

Sample multiplexing-based targeted pathway proteomics with real-time analytics reveals the impact of genetic variation on protein expression.

Yu Q, Liu X, Keller MP, Navarrete-Perea J, Zhang T, Fu S, Vaites LP, Shuken SR, Schmid E, Keele GR, Li J, Huttlin EL, Rashan EH, Simcox J, Churchill GA, Schweppe DK, Attie AD, Paulo JA, Gygi SP.

Nat Commun. 2023 Feb 2; 1(14)555

Oligonucleotide-directed proximity-interactome mapping (O-MAP): A unified method for discovering RNA-interacting proteins, transcripts and genomic loci in situ.

Tsue AF, Kania EE, Lei DQ, Fields R, McGann CD, Hershberg E, Deng X, Kihiu M, Ong SE, Disteche CM, Kugel S, Beliveau BJ, Schweppe DK, Shechner DM.

bioRxiv. 2023 Jan 20;

A proteome-wide atlas of drug mechanism of action.

Mitchell DC, Kuljanin M, Li J, Van Vranken JG, Bulloch N, Schweppe DK, Huttlin EL, Gygi SP.

Nat Biotechnol. 2023 Jan 2;

Conditional Fragment Ion Probabilities Improve Database Searching for Nonmonoisotopic Precursors.

O'Brien JJ, Gadzuk-Shea M, Seitzer PM, Rad R, McAllister FE, Schweppe DK.

J Proteome Res. 2023 Feb 3; 2(22)334-342

Reducing Peptide Sequence Bias in Quantitative Mass Spectrometry Data with Machine Learning.

Dincer AB, Lu Y, Schweppe DK, Oh S, Noble WS.

J Proteome Res. 2022 Jul 1; 7(21)1771-1782

PubMed Collection Link

Accepting Students For:

Rotation, Spring

Permanent

Lab Information

Location: University of Washington
Building: Foege Building
Room: S-041
Box: Box 355065
Phone: 206-616-6031
https://www.schweppelab.org/