Charles Asbury
casbury@uw.eduUniversity of Washington,
Biophysical and Structural Biology
Cancer Biology
Gene Expression, Cell Cycle & Chromosome Biology
Molecular basis of chromosome segregation during cell division
Faculty Contact Information
Lab Information
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Publications
The following publications were retrieved from PubMed:
Tight bending of the Ndc80 complex provides intrinsic regulation of its binding to microtubules.
Scarborough EA, Davis TN, Asbury CL.
Elife. 2019 May 2; (8)
Relax, Kinetochores Are Exquisitely Sensitive to Tension.
Larson JD, Asbury CL.
Dev Cell. 2019 Apr 8; 1(49)5-7
The kinetoplastid kinetochore protein KKT4 is an unconventional microtubule tip-coupling protein.
Llauró A, Hayashi H, Bailey ME, Wilson A, Ludzia P, Asbury CL, Akiyoshi B.
J Cell Biol. 2018 Nov 5; 11(217)3886-3900
Jung SR, Deng Y, Kushmerick C, Asbury CL, Hille B, Koh DS.
Proc Natl Acad Sci U S A. 2018 Jun 19; 25(115)E5706-E5715
Helgeson LA, Zelter A, Riffle M, MacCoss MJ, Asbury CL, Davis TN.
Proc Natl Acad Sci U S A. 2018 Mar 13; 11(115)2740-2745
Driver JW, Geyer EA, Bailey ME, Rice LM, Asbury CL.
Elife. 2017 Jun 19; (6)
Direct measurement of the strength of microtubule attachment to yeast centrosomes.
Fong KK, Sarangapani KK, Yusko EC, Riffle M, Llauró A, Graczyk B, Davis TN, Asbury CL.
Mol Biol Cell. 2017 Jul 7; 14(28)1853-1861
Anaphase A: Disassembling Microtubules Move Chromosomes toward Spindle Poles.
Asbury CL
Biology (Basel). 2017 Feb 17; 1(6)
The Ndc80 complex bridges two Dam1 complex rings.
Kim JO, Zelter A, Umbreit NT, Bollozos A, Riffle M, Johnson R, MacCoss MJ, Asbury CL, Davis TN.
Elife. 2017 Feb 13; (6)
Deng Y, Asbury CL.
Methods Mol Biol. 2017; (1486)437-467
A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments.
Miller MP, Asbury CL, Biggins S.
Cell. 2016 Jun 2; 6(165)1428-1439
Data Analysis for Total Internal Reflection Fluorescence Microscopy.
Asbury CL
Cold Spring Harb Protoc. 2016 May 2; 5(2016)
Jung SR, Seo JB, Deng Y, Asbury CL, Hille B, Koh DS.
J Gen Physiol. 2016 Mar; 3(147)255-71
Force is a signal that cells cannot ignore.
Yusko EC, Asbury CL.
Mol Biol Cell. 2014 Nov 15; 23(25)3717-25
Umbreit NT, Miller MP, Tien JF, Ortolá JC, Gui L, Lee KK, Biggins S, Asbury CL, Davis TN.
Nat Commun. 2014 Sep 19; (5)4951
Tien JF, Umbreit NT, Zelter A, Riffle M, Hoopmann MR, Johnson RS, Fonslow BR, Yates JR 3rd, MacCoss MJ, Moritz RL, Asbury CL, Davis TN.
Genetics. 2014 Dec; 4(198)1483-93
Sister kinetochores are mechanically fused during meiosis I in yeast.
Sarangapani KK, Duro E, Deng Y, Alves Fde L, Ye Q, Opoku KN, Ceto S, Rappsilber J, Corbett KD, Biggins S, Marston AL, Asbury CL.
Science. 2014 Oct 10; 6206(346)248-51
Catch and release: how do kinetochores hook the right microtubules during mitosis?
Sarangapani KK, Asbury CL.
Trends Genet. 2014 Apr; 4(30)150-9
Measuring kinetochore-microtubule interaction in vitro.
Driver JW, Powers AF, Sarangapani KK, Biggins S, Asbury CL.
Methods Enzymol. 2014; (540)321-37
Tien JF, Fong KK, Umbreit NT, Payen C, Zelter A, Asbury CL, Dunham MJ, Davis TN.
Genetics. 2013 Sep; 1(195)159-70
Sarangapani KK, Akiyoshi B, Duggan NM, Biggins S, Asbury CL.
Proc Natl Acad Sci U S A. 2013 Apr 30; 18(110)7282-7
The Ndc80 kinetochore complex directly modulates microtubule dynamics.
Umbreit NT, Gestaut DR, Tien JF, Vollmar BS, Gonen T, Asbury CL, Davis TN.
Proc Natl Acad Sci U S A. 2012 Oct 2; 40(109)16113-8
Stumpff J, Wagenbach M, Franck A, Asbury CL, Wordeman L.
Dev Cell. 2012 May 15; 5(22)1017-29
Stumpff J, Du Y, English CA, Maliga Z, Wagenbach M, Asbury CL, Wordeman L, Ohi R.
Mol Cell. 2011 Sep 2; 5(43)764-75
Tension directly stabilizes reconstituted kinetochore-microtubule attachments.
Akiyoshi B, Sarangapani KK, Powers AF, Nelson CR, Reichow SL, Arellano-Santoyo H, Gonen T, Ranish JA, Asbury CL, Biggins S.
Nature. 2010 Nov 25; 7323(468)576-9
Kinetochores' gripping feat: conformational wave or biased diffusion?
Asbury CL, Tien JF, Davis TN.
Trends Cell Biol. 2011 Jan; 1(21)38-46
Kip3, the yeast kinesin-8, is required for clustering of kinetochores at metaphase.
Wargacki MM, Tay JC, Muller EG, Asbury CL, Davis TN.
Cell Cycle. 2010 Jul 1; 13(9)2581-8
Tien JF, Umbreit NT, Gestaut DR, Franck AD, Cooper J, Wordeman L, Gonen T, Asbury CL, Davis TN.
J Cell Biol. 2010 May 17; 4(189)713-23
Reconstitution and functional analysis of kinetochore subcomplexes.
Gestaut DR, Cooper J, Asbury CL, Davis TN, Wordeman L.
Methods Cell Biol. 2010; (95)641-56
Franck AD, Powers AF, Gestaut DR, Davis TN, Asbury CL.
Methods. 2010 Jun; 2(51)242-50
Cooper JR, Wagenbach M, Asbury CL, Wordeman L.
Nat Struct Mol Biol. 2010 Jan; 1(17)77-82
On the origin of kinesin limping.
Fehr AN, Gutiérrez-Medina B, Asbury CL, Block SM.
Biophys J. 2009 Sep 16; 6(97)1663-70
Powers AF, Franck AD, Gestaut DR, Cooper J, Gracyzk B, Wei RR, Wordeman L, Davis TN, Asbury CL.
Cell. 2009 Mar 6; 5(136)865-75
Gestaut DR, Graczyk B, Cooper J, Widlund PO, Zelter A, Wordeman L, Asbury CL, Davis TN.
Nat Cell Biol. 2008 Apr; 4(10)407-14
Chromosome bi-orientation: Euclidian euploidy.
Stumpff J, Asbury CL.
Curr Biol. 2008 Jan 22; 2(18)R81-3
Kinesin steps do not alternate in size.
Fehr AN, Asbury CL, Block SM.
Biophys J. 2008 Feb 1; 3(94)L20-2
Franck AD, Powers AF, Gestaut DR, Gonen T, Davis TN, Asbury CL.
Nat Cell Biol. 2007 Jul; 7(9)832-7
The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement.
Asbury CL, Gestaut DR, Powers AF, Franck AD, Davis TN.
Proc Natl Acad Sci U S A. 2006 Jun 27; 26(103)9873-8
Uy JL, Asbury CL, Petersen TW, van den Engh G.
Cytometry A. 2004 Sep; 1(61)18-25
Kinesin moves by an asymmetric hand-over-hand mechanism.
Asbury CL, Fehr AN, Block SM.
Science. 2003 Dec 19; 5653(302)2130-4
Probing the kinesin reaction cycle with a 2D optical force clamp.
Block SM, Asbury CL, Shaevitz JW, Lang MJ.
Proc Natl Acad Sci U S A. 2003 Mar 4; 5(100)2351-6
Trapping of DNA by dielectrophoresis.
Asbury CL, Diercks AH, van den Engh G.
Electrophoresis. 2002 Aug; 16(23)2658-66
An automated two-dimensional optical force clamp for single molecule studies.
Lang MJ, Asbury CL, Shaevitz JW, Block SM.
Biophys J. 2002 Jul; 1(83)491-501
Polarization of scatter and fluorescence signals in flow cytometry.
Asbury CL, Uy JL, van den Engh G.
Cytometry. 2000 Jun 1; 2(40)88-101
Trapping of DNA in nonuniform oscillating electric fields.
Asbury CL, van den Engh G.
Biophys J. 1998 Feb; 2 Pt 1(74)1024-30
Peattie RA, Asbury CL, Bluth EI, Riehle TJ.
J Ultrasound Med. 1996 Oct; 10(15)689-96
Steady flow in models of abdominal aortic aneurysms. Part I: Investigation of the velocity patterns.
Peattie RA, Asbury CL, Bluth EI, Ruberti JW.
J Ultrasound Med. 1996 Oct; 10(15)679-88
Fluorescence spectra of DNA dyes measured in a flow cytometer.
Asbury CL, Esposito R, Farmer C, van den Engh G.
Cytometry. 1996 Jul 1; 3(24)234-42
Experimental investigation of steady flow in rigid models of abdominal aortic aneurysms.
Asbury CL, Ruberti JW, Bluth EI, Peattie RA.
Ann Biomed Eng. 1995 Jan-Feb; 1(23)29-39
Research Summary
Our laboratory studies the mitotic spindle, an exquisite molecular machine that organizes and separates duplicated chromosomes during cell division, thereby ensuring equal partitioning of the genetic material. To uncover how this machine operates, we are reconstituting spindle functions using pure components and applying new biophysical tools for manipulating and tracking individual molecules, such as laser trapping and ultrasensitive fluorescence microscopy.