Research Summary
We use cryo-EM to study the structures of large self assembling macromolecular complexes, including enzyme polymers that regulate cellular metabolism, cytoskeletal structures that organize the intracellular space, and piston-like protein machines that generate force to burst membranes as part of intercellular warfare. In each of these systems protein-protein interactions drive creation of large cellular structures with unique functional properties. We complement our structural work with functional studies using a broad array of biophysical, biochemical, and cell biological approaches.
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Publications
The following publications were retrieved from PubMed:
Computational design of transmembrane pores.
Xu C, Lu P, Gamal El-Din TM, Pei XY, Johnson MC, Uyeda A, Bick MJ, Xu Q, Jiang D, Bai H, Reggiano G, Hsia Y, Brunette TJ, Dou J, Ma D, Lynch EM, Boyken SE, Huang PS, Stewart L, DiMaio F, Kollman JM, Luisi BF, Matsuura T, Catterall WA, Baker D.
Nature. 2020 Sep; 7823(585)129-134
