Jackie Lang

Gene Expression, Cell Cycle & Chromosome Biology

Entry Quarter: Autumn 2011

Application of an in vitro kinetochore assembly method to explore the role of the Aurora B mitotic kinase during assembly

Defense date: 05/21/2018
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Publications Link

Research Summary

I have developed a cell-free method for assembling yeast kinetochores that contain all kinetochore subcomplexes and exhibit microtubule-binding activity. Furthermore, the in vitro assembly appears to be stepwise and is dependent on the CENP-A chaperone. Initially, we have been using this technique to explore a novel role for the mitotic kinase Aurora B in promoting kinetochore assembly. We have also been using this method to map the hierarchy of inner kinetochore assembly by performing in vitro assembly in the absence of individual proteins.

Lab Information

Box: A2-168