Gene Expression, Cell Cycle & Chromosome Biology
Regulation of centromeric histone localization in budding yeast
The following publications were retrieved from PubMed:
Hildebrand EM, Dekker J.
Trends Biochem Sci. 2020 May; 5(45)385-396
Deyter GM, Hildebrand EM, Barber AD, Biggins S.
Genetics. 2017 Jan; 1(205)113-124
Hildebrand EM, Biggins S.
PLoS Genet. 2016 Mar; 3(12)e1005930
My PhD research was focused on the question of how the centromeric histone variant (CENP-A/Cse4) is regulated so that it is bound only to the centromere and not to other parts of the genome. I used ChIP-seq to measure the genome-wide mislocalization pattern of CENP-A/Cse4 in the absence of Psh1, the E3 ubiquitin ligase that targets CENP-A/Cse4 for degradation and I also investigated other chromatin-based processes that control CENP-A/Cse4 localization to prevent ectopic nucleosome formation on chromosome arms.