student

Anja Ollodart

anjao@uw.edu

University of Washington,

Genome Sciences

Genetics, Genomics & Evolution

Entry Quarter: Autumn 2015

Lab Information

Advisor: Maitreya Dunham

Publications

The following publications were retrieved from PubMed:

Phenotypic and Genotypic Consequences of CRISPR/Cas9 Editing of the Replication Origins in the rDNA of <i>Saccharomyces cerevisiae</i>.

Sanchez JC, Ollodart A, Large CRL, Clough C, Alvino GM, Tsuchiya M, Crane M, Kwan EX, Kaeberlein M, Dunham MJ, Raghuraman MK, Brewer BJ.

Genetics. 2019 Jul 10;

Cooperation between distinct viral variants promotes growth of H3N2 influenza in cell culture.

Xue KS, Hooper KA, Ollodart AR, Dingens AS, Bloom JD.

Elife. 2016 Mar 15; (5)e13974

Mycobacterium tuberculosis supports protein tyrosine phosphorylation.

Kusebauch U, Ortega C, Ollodart A, Rogers RS, Sherman DR, Moritz RL, Grundner C.

Proc Natl Acad Sci U S A. 2014 Jun 24; 25(111)9265-70

Mycobacterium tuberculosis Ser/Thr protein kinase B mediates an oxygen-dependent replication switch.

Ortega C, Liao R, Anderson LN, Rustad T, Ollodart AR, Wright AT, Sherman DR, Grundner C.

PLoS Biol. 2014 Jan; 1(12)e1001746

Mycobacterium tuberculosis Rv2179c protein establishes a new exoribonuclease family with broad phylogenetic distribution.

Abendroth J, Ollodart A, Andrews ES, Myler PJ, Staker BL, Edwards TE, Arcus VL, Grundner C.

J Biol Chem. 2014 Jan 24; 4(289)2139-47

Research Summary

My project focuses on developing a high-throughput and multiplexed way of determining mutation rate of genetically diverse microbial organisms using continuous culture. The first application of this method is to do a screen of variants in the DNA Mismatch repair pathway in yeast. Variants within this pathway are responsible for Lynch syndrome – a predisposition to a variety of cancers including colon cancer. A first pass at their functionality in yeast will allow a more focused view on potentially pathogenic variants of this pathway.