faculty

Kelly Lee

kklee@uw.edu

University of Washington, 

Biophysical and Structural Biology

Microbiology, Infection & Immunity

VIRUS STRUCTURE, DYNAMICS AND FUNCTION. MEMBRANE FUSION.

Faculty Contact Information

Building: Health Sciences Building Room: H-172J Box: 357610 Phone: 206-616-3972 http://faculty.washington.edu/kklee/Welcome.html

Lab Information

Location: University of Washington Building: Health Sciences Building Room: H-053/H-059 Box: 357610 Phone: 206-616-3496 http://faculty.washington.edu/kklee/Welcome.html

Accepting Students For:

Rotation, Autumn
Rotation, Spring
Rotation, Summer
Rotation, Winter
Permanent

Publications

Tracking Higher Order Protein Structure by Hydrogen-Deuterium Exchange Mass Spectrometry

Benhaim M, Lee KK, Guttman M.

Protein and peptide letters. Forthcoming;

My Bibliography [journal]

Influenza Virus-Liposome Fusion Studies Using Fluorescence Dequenching and Cryo-electron Tomography.

Gui L, Lee KK.

Methods in molecular biology (Clifton, N.J.). 2018; 1836:261-279. NIHMSID: NIHMS953012

PubMed [journal]
PMID:
30151578
PMCID:
PMC6116526

Methods in Molecular Biology

Gui L, Lee KK.

Yamauchi Y, editor. New York: Springer; 2018. Influenza Virus-Liposome Fusion Studies using Fluoresence Dequenching and Cryo-Electron Tomography

My Bibliography [book]

Dissection of epitope-specific mechanisms of neutralization of influenza virus by intact IgG and Fab fragments.

Williams JA, Gui L, Hom N, Mileant A, Lee KK.

Journal of virology. 2017;

PubMed [journal]
PMID:
29263254
PMCID:
PMC5827376

Computational design of trimeric influenza-neutralizing proteins targeting the hemagglutinin receptor binding site.

Strauch EM, Bernard SM, La D, Bohn AJ, Lee PS, Anderson CE, Nieusma T, Holstein CA, Garcia NK, Hooper KA, Ravichandran R, Nelson JW, Sheffler W, Bloom JD, Lee KK, Ward AB, Yager P, Fuller DH, Wilson IA, Baker D.

Nature biotechnology. 2017; 35(7):667-671. NIHMSID: HHMIMS878279

PubMed [journal]
PMID:
28604661
PMCID:
PMC5512607

Research Summary

Viruses undergo dynamic structural reorganizations at many critical stages of their life cycles including during host cell invasion, membrane fusion, genome expulsion, assembly, and cell egress. The changes often involve concerted changes among hundreds of protein components and in the case of enveloped viruses, membranes as well. We use biophysical, structural, and biochemical techniques including cryo-EM, hydrogen/deuterium-exchange with mass spectrometry (HDX-MS), and small-angle X-ray scattering (SAXS) to understand these dynamic processes.