Liangcai Gu
gulc@uw.eduUniversity of Washington,
Biophysical and Structural Biology
Microbiology, Infection & Immunity
Protein interaction profiling and engineering using in situ sequencing technologies
Faculty Contact Information
Lab Information
Accepting Students For:
Publications
The following publications were retrieved from PubMed:
Structural Basis for Cyclopropanation by a Unique Enoyl-Acyl Carrier Protein Reductase.
Khare D, Hale WA, Tripathi A, Gu L, Sherman DH, Gerwick WH, Håkansson K, Smith JL.
Structure. 2015 Dec 1; 12(23)2213-2223
Multiplex single-molecule interaction profiling of DNA-barcoded proteins.
Gu L, Li C, Aach J, Hill DE, Vidal M, Church GM.
Nature. 2014 Nov 27; 7528(515)554-7
Chang Z, Gu L.
Sci China Life Sci. 2014 Oct; 10(57)1039-40
Improved cell-free RNA and protein synthesis system.
Li J, Gu L, Aach J, Church GM.
PLoS One. 2014; 9(9)e106232
Structure and activity of DmmA, a marine haloalkane dehalogenase.
Gehret JJ, Gu L, Geders TW, Brown WC, Gerwick L, Gerwick WH, Sherman DH, Smith JL.
Protein Sci. 2012 Feb; 2(21)239-48
Busche A, Gottstein D, Hein C, Ripin N, Pader I, Tufar P, Eisman EB, Gu L, Walsh CT, Sherman DH, Löhr F, Güntert P, Dötsch V.
ACS Chem Biol. 2012 Feb 17; 2(7)378-86
Gu L, Eisman EB, Dutta S, Franzmann TM, Walter S, Gerwick WH, Skiniotis G, Sherman DH.
Angew Chem Int Ed Engl. 2011 Mar 14; 12(50)2795-8
Gehret JJ, Gu L, Gerwick WH, Wipf P, Sherman DH, Smith JL.
J Biol Chem. 2011 Apr 22; 16(286)14445-54
Conformational switch triggered by alpha-ketoglutarate in a halogenase of curacin A biosynthesis.
Khare D, Wang B, Gu L, Razelun J, Sherman DH, Gerwick WH, Håkansson K, Smith JL.
Proc Natl Acad Sci U S A. 2010 Aug 10; 32(107)14099-104
Polyketide decarboxylative chain termination preceded by o-sulfonation in curacin a biosynthesis.
Gu L, Wang B, Kulkarni A, Gehret JJ, Lloyd KR, Gerwick L, Gerwick WH, Wipf P, Håkansson K, Smith JL, Sherman DH.
J Am Chem Soc. 2009 Nov 11; 44(131)16033-5
Metamorphic enzyme assembly in polyketide diversification.
Gu L, Wang B, Kulkarni A, Geders TW, Grindberg RV, Gerwick L, Håkansson K, Wipf P, Smith JL, Gerwick WH, Sherman DH.
Nature. 2009 Jun 4; 7247(459)731-5
New tricks from ancient algae: natural products biosynthesis in marine cyanobacteria.
Jones AC, Gu L, Sorrels CM, Sherman DH, Gerwick WH.
Curr Opin Chem Biol. 2009 Apr; 2(13)216-23
Sharp K, Arthur KE, Gu L, Ross C, Harrison G, Gunasekera SP, Meickle T, Matthew S, Luesch H, Thacker RW, Sherman DH, Paul VJ.
Appl Environ Microbiol. 2009 May; 9(75)2879-88
GNAT-like strategy for polyketide chain initiation.
Gu L, Geders TW, Wang B, Gerwick WH, Håkansson K, Smith JL, Sherman DH.
Science. 2007 Nov 9; 5852(318)970-4
Geders TW, Gu L, Mowers JC, Liu H, Gerwick WH, Håkansson K, Sherman DH, Smith JL.
J Biol Chem. 2007 Dec 7; 49(282)35954-63
Gu L, Jia J, Liu H, Håkansson K, Gerwick WH, Sherman DH.
J Am Chem Soc. 2006 Jul 19; 28(128)9014-5
Fu X, Liu C, Liu Y, Feng X, Gu L, Chen X, Chang Z.
Biochem Biophys Res Commun. 2003 Oct 17; 2(310)412-20
Abulimiti A, Fu X, Gu L, Feng X, Chang Z.
J Mol Biol. 2003 Feb 28; 4(326)1013-23
Gu L, Abulimiti A, Li W, Chang Z.
J Mol Biol. 2002 May 31; 2(319)517-26
Research Summary
We use quantitative protein interaction profiling to understand molecular recognition and guide computational protein design. We develop protein interaction sequencing technologies by coupling protein barcoding and in situ sequencing techniques to measure single-molecule proteins and complexes in massively parallel. Our current research is focused in three major areas: (1) engineering of ligand-responsive protein assemblies, (2) human protein interactome profiling and drug screening, and (3) functional profiling of antigen receptors.