faculty

Liangcai Gu

gulc@uw.edu

Assistant Professor

University of Washington

Biochemistry

Biophysical and Structural Biology

Microbiology, Infection & Immunity

Protein interaction profiling and engineering using in situ sequencing technologies

Faculty Contact Information

Building: HSB Room: J583 Box: UW Box 357350 Phone: 206-221-0382 Alt Phone: 206-221-7730 http://interactomelab.org/

Research Summary

We use quantitative protein interaction profiling to understand molecular recognition and guide computational protein design. We develop protein interaction sequencing technologies by coupling protein barcoding and in situ sequencing techniques to measure single-molecule proteins and complexes in massively parallel. Our current research is focused in three major areas: (1) engineering of ligand-responsive protein assemblies, (2) human protein interactome profiling and drug screening, and (3) functional profiling of antigen receptors.

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Publications

The following publications were retrieved from PubMed:

Combinatorial Approaches for Efficient Design of Photoswitchable Protein-Protein Interactions as In Vivo Actuators.

Zhang X, Pan Y, Kang S, Gu L.

Front Bioeng Biotechnol. 2022; (10)844405

Defining molecular glues with a dual-nanobody cannabidiol sensor.

Cao S, Kang S, Mao H, Yao J, Gu L, Zheng N.

Nat Commun. 2022 Feb 10; 1(13)815

Synthetic nanobody-functionalized nanoparticles for accelerated development of rapid, accessible detection of viral antigens.

Chen X, Kang S, Ikbal MA, Zhao Z, Pan Y, Zuo J, Gu L, Wang C.

Biosens Bioelectron. 2022 Apr 15; (202)113971

Creating Red Light-Switchable Protein Dimerization Systems as Genetically Encoded Actuators with High Specificity.

Huang Z, Li Z, Zhang X, Kang S, Dong R, Sun L, Fu X, Vaisar D, Watanabe K, Gu L.

ACS Synth Biol. 2020 Dec 18; 12(9)3322-3333

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library.

Gomez-Castillo L, Watanabe K, Jiang H, Kang S, Gu L.

J Vis Exp. 2020 Jan 14; 155

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Lab Information

Location: University of Washington
Building: HSB
Room: J579
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