Liangcai Gu


University of Washington, 

Biophysical and Structural Biology

Microbiology, Infection & Immunity

Protein interaction profiling and engineering using in situ sequencing technologies

Faculty Contact Information

Building: HSB Room: J583 Box: UW Box 357350 Phone: 206-221-0382 Alt Phone: 206-221-7730 http://interactomelab.org/

Lab Information

Location: University of Washington Building: HSB Room: J579 Phone: 206-221-7730 http://interactomelab.org/

Accepting Students For:

Rotation, Autumn
Rotation, Spring
Rotation, Winter


The following publications were retrieved from PubMed:

Structural Basis for Cyclopropanation by a Unique Enoyl-Acyl Carrier Protein Reductase.

Khare D, Hale WA, Tripathi A, Gu L, Sherman DH, Gerwick WH, Håkansson K, Smith JL.

Structure. 2015 Dec 1; 12(23)2213-2223

Multiplex single-molecule interaction profiling of DNA-barcoded proteins.

Gu L, Li C, Aach J, Hill DE, Vidal M, Church GM.

Nature. 2014 Nov 27; 7528(515)554-7

Improved cell-free RNA and protein synthesis system.

Li J, Gu L, Aach J, Church GM.

PLoS One. 2014; 9(9)e106232

Structure and activity of DmmA, a marine haloalkane dehalogenase.

Gehret JJ, Gu L, Geders TW, Brown WC, Gerwick L, Gerwick WH, Sherman DH, Smith JL.

Protein Sci. 2012 Feb; 2(21)239-48

Research Summary

We use quantitative protein interaction profiling to understand molecular recognition and guide computational protein design. We develop protein interaction sequencing technologies by coupling protein barcoding and in situ sequencing techniques to measure single-molecule proteins and complexes in massively parallel. Our current research is focused in three major areas: (1) engineering of ligand-responsive protein assemblies, (2) human protein interactome profiling and drug screening, and (3) functional profiling of antigen receptors.